Fig. 3

Microglial activation following T. gondii infection in 3xTg-AD mice 7 days PI. Activated microglia were identified by increased Iba1 positivity, thickened processes and irregular cell bodies. Microglial activation is shown in the cortex of a 5–6-month-old mice and b 11–12-month-old mice at day 7 PI. c Iba1 staining in the cortex of naïve and infected mice at day 7 PI; insets show representative images from each group. Scale bars show 100 μm. *P < 0.05; **P ≤ 0.01; ***P ≤ 0.001. Secondary comparisons are indicated by ^## P ≤ 0.01 above groups which differ significantly from one another. All data are shown as mean ± SEM