Fig. 3

Teri and MMF preserve neuronal viability. Treatment of human fetal neurons (HFN) with conditioned media of HMC3 microglia co-cultured with HIV vector-transduced monocytoid cells induced cell death after 48 h (a, c) compared to the non-transduced control condition (p < 0.0001; (b)). Neurotoxicity was significantly reduced upon treatment with 10 μM Teri (p < 0.01) (d) and 10 μM (p < 0.05) (e) or 30 μM MMF (p < 0.05). Staining for microtubuli-associated protein (MAP)-2 (green), nuclei (Hoechst S769121, blue) and propidium iodide (red). Pictures are shown in ×20 magnification, the scale bar represents 100 μM. Shown are data generated with conditioned media of three independent experiments performed in triplicates (a). Statistics was performed using one-way ANOVA with Sidak´s multiple comparisons test as post hoc analysis. MAP-2⁺PI⁺ cells were subtracted from MAP-2⁺PI⁻ cells, thus only showing surviving neurons. Data are normalized to the control condition HMC3 + non-transduced monocytoid cells and are shown as mean ± SEM. Significance is shown compared to conditioned media of the HIV vector transduced co-culture condition (“HIV vector”). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001