Fig. 1

Chemokine RNA profiling in the substantia nigra of MPTP mice reveals early CCL2/7/12-CCR2 axis induction. a–b Laser microdissection (LMD) of Nissl-stained substantia nigra pars compacta (SNpc) from mouse midbrain tissue sections, used for TaqMan RT-qPCR profiling of the full chemokine family in MPTP mice. a Showing a SNpc before LMD and b showing multiple SNpcs collected by LMD. (Scale bar; b, 200 μm). c Selected results, showing fold change (P < 0.05; ANOVA with Holm-Sidak test) inductions (RNA) of the CCL2/7/12-CCR2 (ligand receptor) axis at 2, 4, and 7 days (2d/4d/7d) after acute MPTP intoxication, suggesting nigral CCR2⁺ monocyte infiltration. Data are shown as average fold changes compared to saline controls; n = 9 mice per condition, normalized to Hprt1 (for a full list of results, see Additional file 2: Table S1)