Fig. 2

Early but transient CCL2 protein induction within the affected substantia nigra of MPTP mice. a–h Immunofluorescence stainings showing the timecourse of CCL2 protein induction in the SNpc (dotted white line) of MPTP mice, at 12 h (b, f), 24 h (c, g), and 48 h/2 days (d, h) after acute MPTP intoxication, compared to saline injected controls (a, e). CCL2 in red and TH (marking DA neurons) in green. While CCL2 is absent in controls (a, e), first signs of CCL2 induction appear at 12 h (b, f) (arrows), followed by robust induction at 24 h (c, g) (arrows and insets) and reduced induction at 36 h (data not shown) and 48 h/2 days (d, h) (arrows). CCL2 induction does not colocalize with neuronal TH staining (insets in c and g). No CCL2 induction was detected at 4 and 7 days (data not shown). (Scale bar; h, 200 μm)