Table 2 Antibodies used for immunohistochemistry
Target | Antibody type (clone) | Dilution | Pre-treatment | Source |
|---|---|---|---|---|
Amyloid precursor protein (APP) | Mouse mAb (22C11) | 1:2000 | Citrate MW | Chemicon International, USA |
Neuropeptide Y receptor Y1 (NPY-Y1R) | Rabbit polyAb #96106 | 1:1000 | Tris-EDTA MW | CURE/UCLA, USA |
Myelin basic protein (MBP) | Rabbit polyAb | 1:1000 | None | DakoCytomation, Denmark |
MBP peptic fragment 70–89 | Mouse mAb (SMI94) | 1:5000 | Steam | Covance Inc., USA |
Myelin proteolipid protein (PLP) | Mouse mAb (Plpc 1) | 1:500 | Citrate MW | Biozol, Germany |
Myelin oligodendrocyte glycoprotein (MOG) | Rat polyAb | 1:1000 | Citrate MW | [75] |
Myelin-associated glycoprotein (MAG) | Rabbit polyAb | 1:10 | Citrate MW | [76] |
2′,3′-Cyclic-nucleotide 3′-phosphodiesterase (CNPase) | Mouse mAb (SMI91) | 1:200 | Citrate MW | Covance Inc., USA |
Monocytes, activated microglia | Mouse mAb (KiM1P) | 1:5000 | Citrate MW | [77] |
Early-activated macrophages (S100A9) | Rabbit polyAb | 1:500 | Citrate MW | [78] |
Phosphorylated NFs | Mouse mAb (SMI31) | 1:10000 | Citrate MW | Covance Inc., USA |
Non-phosphorylated NFs | Mouse mAb (SMI32) | 1:1000 | Citrate MW | Covance Inc., USA |
Hypo-phosphorylated NFs | Mouse mAb (SMI35) | 1:10000 | Citrate MW | Covance Inc., USA |
NF-low-molecular-weight (NF68) | Mouse mAb (NR4) | 1:100 | Citrate MW | Sigma Chemical Company, USA |
NF-high-molecular-weight (NF200) | Mouse mAb (N52) | 1:400 | Citrate MW | Sigma Chemical Company, USA |
Growth-associated protein 43 (GAP43) | Mouse mAb (9-1E12) | 1:4000 | Citrate MW | Chemicon International, USA |
Synaptophysin (protein p38) | Mouse mAb (SY38) | 1:10 | Citrate MW | DakoCytomation, Denmark |