Fig. 6

Luciferase-3′-untranslated region reporter assay. Interaction of miR-142a-3p with TGFBR1 and miR-142a-5p with TGFBR2 and SOCS1 transcripts were assessed using luciferase assay system. Co-transfection of HEK293T cells containing luciferase-3′UTR construct from TGFBR1 together with miR-142a-3p mimics showed significant suppression of luciferase activity in comparison with cells transfected with a control miRNA sequence (a). miR-142a-5p transfection did not lead to significant suppression of luciferase activity in cells co-transfected with TGFBR2 3′UTR constructs (b). Similar experiments with luciferase-3′UTR from SOCS1 and miR-142a-5p mimics showed suppression of luciferase activity compared with cells transfected a control sequence (c). Firefly luciferase levels were normalized against renilla luciferase, expressed as an internal control in the vector. Data are shown as mean ± SEM, n = 5. Data are from a single experiment representative of three independent experiments. *p < 0.05, **p < 0.01, Student’s t test