Fig. 2
From: Activation of FXR pathway does not alter glial cell function
Loss of FXR does not impact oligodendroglial differentiation in vitro and in vivo. Immunohistochemical staining of WT and FXR Ko mice, either 10 days or 8 weeks old, display no differences in the number of OLIG2+ oligodendroglial cells in the corpus callosum (cc), cerebellum or spinal cord (a, c). In addition, the proportion of mature NOGO A+ oligodendrocytes (b, d) is not altered in FXR Ko mice compared to WT mice. In vitro cultured, primary oligodendrocytes isolated either from the cerebrum (e, f) or cerebellum (g, h) display no significant difference in the Mbp gene expression (e, g) after 24 and 48 h of differentiation or the percentage of PDGFRα+ OPCs or mature MBP+ oligodendrocytes (f, h) after 48 h of differentiation. In vivo: number of cells/mm2 was determined using an ocular grit, n = 4–6 animals, two-tailed Student’s t test; in vitro: n = 3, one-way ANOVA with Bonferroni’s correction, 200 cells per condition and two-tailed Student’s t test, ns >0.05; all images are representative