Fig. 5
Ontology enrichment analyses reveal biological implication of obtained DEGs with microglial processes. C/EBPβfl/fl and LysMCre-C/EBPβfl/fl DEGs where fed into the Genecodis platform for ontology enrichment. a p value and gene percentage annotation graph of the biological process (BP) category from Gene Ontology database (hypergeometric distribution). b p value and gene percentage annotation graph from Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses (hypergeometric distribution). c–e Heatmap of genes annotated with the enriched categories shown above (hierarchical clustering, average linkage, Euclidean distance, scale in SD over normalized gene expression in log2 values). Note the existence of gene clusters with distinct patterns of gene expression changes, e.g. cluster B in f corresponds to lysosome-related genes with reduced expression upon LPS ± IFNγ treatments, in C/EBPβfl/fl microglia that is attenuated in LysMCre-C/EBPβfl/fl microglia. In this figure, WT and KO refer to C/EBPβfl/fl and LysMCre-C/EBPβfl/fl, respectively