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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Myeloid C/EBPβ deficiency reshapes microglial gene expression and is protective in experimental autoimmune encephalomyelitis

Fig. 8

Neuroprotective effect of microglial C/EBPβ deficiency in EAE. ad Time course analysis of C/EBPβ expression in the CNS in EAE. Expression was analyzed in vehicle-treated (CFA) mice and in EAE at 9, 14, 21 and 28 days post-injection (DPI). a, b C/EBPβ expression at mRNA level by qRT-PCR in the thoracic spinal cord (a) and hindbrain (b). c Representative Western blot image of C/EBPβ protein expression in the thoracic spinal cord in EAE. d Quantification of the C/EBPβ protein isoform LAP (n = 4–6 mice/condition). *p < 0.05; ** p < 0.01 compared with CFA. e EAE clinical scores of mice with myeloid C/EBPβ deficiency (LysMCre-C/EBPβfl/fl mice, n = 10) and the two parental control mouse lines (LysMCre, n = 12; C/EBPβfl/fl n = 12). Data show mean ± SEM. ***p < 0.001. f Western blot showing C/EBPβ protein levels in temporal cortex postmortem samples from three non-neurological controls (CT) and three multiple sclerosis (MS) patients. The three C/EBPβ isoforms, Full (~38 kDa), LAP (~35 kDa) and LIP (~21 kDa), are detected in these samples. g Quantification of C/EBPβ Western blots as that in f. Data show mean + SEM, n = 4 controls, n = 5 MS, *p < 0.05; ***p < 0.001

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