Fig. 3
The three human ApoE isoforms serve as agonists for human and murine TREM2. Human ApoE ε2 (inverted green triangle), ε3 (light blue diamond), and ε4 (red triangle) all stimulated human (a) and murine (b) TREM2 receptor signal transduction in a dose-dependent manner. EC50 for human ApoE was 27, 33, and 34 nM for ApoE ε2, ε3, and ε4, respectively. EC50 for murine TREM2 was 40, 54, and 39 for ApoE ε2, ε3, and ε4, respectively. The points depict mean ± SEM of all responses measured in triplicates from five (human TREM2) and three (murine TREM2) independent experiments. Data from each ApoE isoform was normalized individually as described in the “Methods” section. The scale of the x-axis is logarithmic and shows concentration of human ApoE in M