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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: The Alzheimer’s disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway

Fig. 4

Rougly similar level of TREM2 in BWZ-mTREM2 and hTREM2 cells and lower than in mouse brain. a In both the BWZ-mTREM2 and BWZ-hTREM2 reporter cells, the TREM2 constructs harbor a FLAG-tag. A western blot with an antibody detecting the FLAG-tag expression in the reporter cells indirectly showed that the TREM2 level was slightly higher in the BWZ-mTREM2 than in the BWZ-hTREM2 cells, although this was not statistically significant (n = 2 for both cell lines). Protein loading was 30 μg (lanes 1–2) and 50 μg (lanes 3–4). b Western blot detection of murine TREM2 in BWZ-mTREM2 cells (“BWZ-mT2”) compared to a nontransgenic mouse brain (“ntg ms”). Protein loading was 30 μg (lanes 1–2) and 50 μg (lanes 3–4). TREM2 in the nontransgenic mouse brain was also compared to Trem2 knockout mouse brain (“KO ms”). Protein loading was 40 μg in both lanes. c The level of TREM2 protein in the nontransgenic mouse brain was estimated to approximately 1 ng/μg protein by using recombinant mouse TREM2 (“recT2”) as a standard. Mouse brain extract (30 μg total protein) was loaded in lane 1, while 40 and 80 ng pure, recombinant TREM2 was loaded in lanes 2 and 3, respectively

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