Fig. 6

An ApoE-mimetic peptide (amino acids 130–149) reduced binding of human ApoE to human TREM2. An ApoE-mimetic (amino acids 130–149) or a scrambled peptide was allowed to compete with ApoE for binding to immobilized recombinant human TREM2 in the ELISA-based assay. This led to reduced binding of ApoE, isoforms ε2 (inverted green triangle), ε3 (light blue diamond), and ε4 (red triangle), to human TREM2 with K _i of 827, 475, and 1537 nM for ApoE ε2, ε3, and ε4, respectively. The points depict mean ± SEM of binding measured in duplicates from four independent experiments. The data was normalized to maximum (100%), which is binding of ApoE in the absence of a competing peptide, and minimum (0%), which is signal in the absence of ApoE as well as a TREM2-coat. The x-axis shows ApoE-mim149 or scrambled peptide concentration (in M) on a logarithmic scale