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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Lymphocytes have a role in protection, but not in pathogenesis, during La Crosse Virus infection in mice

Fig. 3

CNS-infiltrating T cells are proliferating and activated during LACV infection but do not significantly alter LACV pathogenesis in weanling mice. Splenocytes and CNS-infiltrating a CD4+, Foxp3 T helper, and b CD8+ CD3+ cytotoxic T cells were analyzed for expression of proliferation and activation markers by flow cytometry at the clinical time point (6–7 dpi). Data are presented as percent (%) of either CD4+ or CD8+ T cells positive for the proliferation marker Ki-67, the activation markers CD43, CD11a, GranzymeB, or CD107a or the naïve T cell marker CD62L. c LACV-infected weanling mice were treated with RPMI/10% FBS (control), anti-CD4 or anti-CD8-depleting monoclonal antibodies as described in the “Methods”. Depletions were confirmed by flow cytometry analysis of brain tissue at the clinical time point (5–7 dpi). Data are a summary plot of two independent experiments with 4–12 mice per group. d 18 vehicle control-, 11 anti-CD8-, and 8 anti-CD4-treated mice were followed for the development of clinical signs of neurological disease. Statistical analysis was completed using the Mantel-Cox log-rank test

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