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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Inflammatory signature of cerebellar neurodegeneration during neonatal hyperbilirubinemia in Ugt1 -/- mouse model

Fig. 3

Bilirubin neurotoxicity activates astrocytes and microglia cells. a WB analysis of total cerebellum protein extract using an anti-GFAP antibody at indicated time points of WT and Ugt1 -/- mice. b Representative fluorescent immunohistochemistry of cerebellar sections from WT and Ugt1 -/- mice using anti-GFAP antibody (red) to detect astrocytes, co-stained with an anti-calbindin antibody (green) to highlight PCs. Scale bar 50 μm. c WB analysis of total cerebellum protein extracts using an anti-Iba1 antibody at P8 and P10 of WT and Ugt1 -/- mice. d Representative fluorescent immunohistochemistry of cerebellar sections from WT and Ugt1 -/- mice using an anti-Iba1 antibody (green) to detect microglia, co-stained with an anti-GFAP antibody (red) to highlight astrocytes. Scale bar 50 μm. For all the experiments, values represent the mean ± SD. Students t test, *p < 0.05. The number of WT and Ugt1 -/- was ≥3 in all the experiments and time points. For WB analysis, β-tubulin was used as loading control. For IF, Hoechst dye (blue) was used to mark nuclei

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