Fig. 8

Bilirubin triggers autophagy in the cerebellum of Ugt1 ^-/- mice. WB analysis of total cerebellum protein extracts using an anti-LC3 antibody at P5, P8 and P10 of WT and Ugt1 ^-/- mice. β-tubulin was used as a loading control. Lower panel: densitometric quantification of the bands; results are expressed as the mean of the LC3 II/I ratio. Student’s t test, ***p < 0.001. P5: WT n = 4, Ugt1 ^-/- n = 4; P8: WT n = 4, Ugt1 ^-/- n = 4; P10: WT n = 3; Ugt1 ^-/- n = 5