Fig. 3

Overexpression of Sirt1 attenuates astrocyte activation in vitro and in vivo. Overexpression of Sirt1 significantly reduced GFAP expression compared with transfection control in vitro (a), n = 6/group, **P < 0.01 vs. the control group; ^## P < 0.01 vs. IL-1β-con; and in vivo (b), *P < 0.05 and **P < 0.01 vs. the sham group; ^# P < 0.05 and ^## P < 0.01 vs. the DMSO group. Immunofluorescence staining revealed that overexpression of Sirt1 improved the signs of astrocyte hypertrophy by immunodetection of GFAP in vitro (c, green) and in vivo (d, red)