Fig. 6

Inhibiting MAPK pathway activation increases Sirt1 expression and alleviates astrocyte activation. a Levels of Sirt1 and GFAP protein in primary astrocytes after IL-1β stimulation in vitro. b Inhibiting MAPK pathway activation significantly increases Sirt1 expression but alleviates GFAP expression in vivo. n = 6/group, *P < 0.05 and **P < 0.01 vs. the DMSO group. Immunofluorescent image of GFAP in vitro and in vivo was showed in (c) and (d). Scale bar = 50 μm