Fig. 3

STAT1 activation is attenuated in NOX2^−/− BMDMs, whereas STAT6 activation is unchanged. (a, b) Protein expression of phosphorylated STAT1 and phosphorylated STAT6 in response to LPS/IL-4 stimulation was assessed by western immunoblotting. (c) LPS/IL-4 (both 10 ng/ml; 24 h) increased pSTAT1 expression (^** P ^* < 0.001, ANOVA) in WT BMDMs. In contrast, LPS/IL-4-induced increase in pSTAT1 was significantly reduced in NOX2^−/− BMDMs (⁺ P < 0.05, WT LPS/IL-4 vs. NOX2^−/− LPS/IL-4; ANOVA). (d) LPS/IL-4 significantly increased pSTAT6 (^*** P < 0.001; ANOVA) in WT and NOX2^−/− BMDMs, but no genotype related differences were observed. All data are expressed as means (± SEM n = 5)