Fig. 4

IL-10 production and downstream effectors are elevated in NOX2^−/− BMDMs in response to LPS/IL-4. (a) LPS/IL-4 (both 10 ng/ml; 24 h) stimulation significantly increased IL-10 protein concentration in WT and NOX2^−/− BMDMs (^*** P < 0.001; ANOVA). The LPS/IL-4-induced increase in IL-10 concentration was significantly increased in NOX2^−/− BMDMs compared with WT BMDMs (⁺⁺⁺p < 0.001, WT LPS/IL-4 vs. NOX2^−/− LPS/IL-4; ANOVA). (b, c) Protein expression of phosphorylated STAT3 was significantly increased in WT and NOX2^−/− BMDMs following LPS/IL-4 stimulation (^*** P < 0.001; ANOVA; representative western immunoblot shown in b). LPS/IL-4-induced pSTAT3 expression was significantly increased in NOX2^−/− BMDMs compared with WT BMDMs (⁺⁺⁺ P < 0.001, WT LPS/IL-4 vs. NOX2^−/− LPS/IL-4; ANOVA). (d) LPS/IL-4 significantly increased IL-4Rα mRNA expression NOX2^−/− BMDMs (^*** P < 0.001; ANOVA). (e) SOCS3 mRNA expression was significantly increased in WT and NOX2^−/− BMDMs following LPS/IL-4 stimulation (^*** P < 0.001; ANOVA). LPS/IL-4-induced SOCS3 expression was significantly increased NOX2^−/− BMDMs compared with WT BMDMs (⁺⁺⁺ P < 0.001, WT LPS/IL-4 vs. NOX2^−/− LPS/IL-4; ANOVA). All data are expressed as means (± SEM, n = 6)