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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Grape seed-derived procyanidins alleviate gout pain via NLRP3 inflammasome suppression

Fig. 1

Procyanidins suppressed MSU-induced NLRP3 inflammasome activation in RAW macrophages. a The cells were stimulated by LPS (1 μg/ml) for 6 h and then stimulated with MSU crystals for another 6 h. Procyanidins were added 20 min before LPS administration. Western blot samples were prepared from the supernatant (n = 4). b The cell extracts were collected at 12 h following LPS treatment from Raw 264.7 cells; procyanidins were added 20 min before LPS (n = 4). c The cells were stimulated by LPS (1 μg/ml) for 6 h and then stimulated with MSU crystals for another 6 h. Procyanidins (10 μM) were added 20 min before LPS. The supernatant (SN) and cell lysis fractions (Input) of Raw 264.7 cells were collected respectively (n = 4). *p < 0.05, **p < 0.01, ***p < 0.001 vs. naive; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the MSU-treated group

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