Fig. 3

Procyanidins suppressed MSU-induced gout pain in mice. Mice were treated with various doses of procyanidins (PO) 20 min before the injection of MSU crystals (0.5 mg/10 μL). a Mechanical allodynia was performed to evaluate the effect of procyanidins (n = 8). b Time course of changes in MSU-induced ankle swelling (n = 8). c Representative photographs of mouse ankles at 24 h following MSU injection. Bar: 5 mm (d) hematoxylin- and eosin-stained sections of the ankle joints obtained 24 h after MSU injection. Bar: 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001 vs. normal; ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 vs. the MSU-treated group