Fig. 3
From: TNF-α promotes extracellular vesicle release in mouse astrocytes through glutaminase
TNF-α induces ROS generation in mouse astrocytes. Mouse astrocytes were pretreated with 1 mM L-DON (a) or 10 μM BPTES (b, c) and 10 mM NAC for 30 min, and then treated with TNF-α (50 ng/ml). After 6 h (L-DON) or 48 h (BPTES, NAC) treatment, cells were stained by loading with 10 μM DCFH-DA for 30 min at 37 °C. Cells were washed with serum-free culture medium, and then subjected to fluorescence microscope (a, b) and fluorescent microplate reader (c). All results were representative of three independent experiments (n = 3)