Fig. 8

Effect of BMP7 on gliosis in absence of microglia—localization of gliosis markers. Mouse retinal sections from eyes injected with vehicle or BMP7 were labeled for gliosis markers GFAP (A, B (a, d, g, j)), S100-β (A, B (b, e, h, k)), and NCAN (A, B (c, f, j, l)). Mice kept on the PLX diet did not show an increase in label for the gliosis markers GFAP and S100-β BMP7 or vehicle-injected retina 3 and 7 days postinjection (A, B (g, h, j, k)). NCAN label appeared to be similar in the BMP7 injected and the respective age-matched vehicle controls in mice kept on the PLX chow (A, B (i, l)). Mice kept on the control chow and injected with BMP7 clearly showed an increase in GFAP, S100-β, and NCAN levels 7 days postinjection, when compared to their respective vehicle control (B (a–f)). Three days postinjection, there is an increase in GFAP and NCAN label in BMP7-injected retinas in comparison to the respective vehicle control-injected retinas (A (a, c, d, f)). When comparing mice kept on control chow or the PLX chow, there is an increase in GFAP and S100-β label in the mice kept on control chow in comparison to the mice kept on the PLX chow, 7 days post BMP7 injection (B (d, e, j, k)). GFAP and S100-β label in mice kept on control chow and PLX chow appears to be similar in the BMP7-injected retinas, 3 days postinjection (A (d, e, j, k)). Magnification bar in (B, (c)) = 50 μm, for images A, B (a–l)