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Table 2 List of antibodies

From: Microglia activation is essential for BMP7-mediated retinal reactive gliosis

Marker Company If concentration Flatmount concentration Western blot concentration
IBA1 WAKO 1:500 1:250  
PU.1 CELL SIGNALING 1:100   
SOX9 MILLIPORE 1:500   
GαT1 SANTA CRUZ   1:100  
CALBINDIN SIGMA 1:250   
CHX10 EXALPHA 1:500   
BRN3A CHEMICON 1:250   
GFAP DAKO (polyclonal) 1:250 1:100  
GFAP DAKO (monoclonal)    1:1000
S100β ABCAM 1:300   1:1000
NCAN R&D SYSTEMS 1:100   
TXNIP SANTA CRUZ    1:250
phospho-SMAD1/5/9 CELL SIGNALING 1:100   
phsopho-TAK ABCAM 1:500   
β - TUBULIN SIGMA    1:1000
GFP THERMO SCIENTIFIC 1:150