Table 2 List of antibodies
From: Microglia activation is essential for BMP7-mediated retinal reactive gliosis
| Marker | Company | If concentration | Flatmount concentration | Western blot concentration |
|---|---|---|---|---|
| IBA1 | WAKO | 1:500 | 1:250 | |
| PU.1 | CELL SIGNALING | 1:100 | ||
| SOX9 | MILLIPORE | 1:500 | ||
| GαT1 | SANTA CRUZ | 1:100 | ||
| CALBINDIN | SIGMA | 1:250 | ||
| CHX10 | EXALPHA | 1:500 | ||
| BRN3A | CHEMICON | 1:250 | ||
| GFAP | DAKO (polyclonal) | 1:250 | 1:100 | |
| GFAP | DAKO (monoclonal) | 1:1000 | ||
| S100β | ABCAM | 1:300 | 1:1000 | |
| NCAN | R&D SYSTEMS | 1:100 | ||
| TXNIP | SANTA CRUZ | 1:250 | ||
| phospho-SMAD1/5/9 | CELL SIGNALING | 1:100 | ||
| phsopho-TAK | ABCAM | 1:500 | ||
| β - TUBULIN | SIGMA | 1:1000 | ||
| GFP | THERMO SCIENTIFIC | 1:150 |