Fig. 4
From: Microglial depletion alters the brain neuroimmune response to acute binge ethanol withdrawal
Acute ethanol changes BV2 microglial cytokine gene expression in vitro. Time course of cytokine expression in ethanol-treated BV2 cells: Microglia-like BV2 cells were treated with ethanol (85 mM) and the ethanol evaporated over time. Media ethanol concentrations (MECs) were measured (dashed line—left axis) and mRNA was collected from the BV2 cells. mRNA levels of a TNFα b Ccl2 c IL-1ra, and d IL-4 in ethanol-treated cells were normalized to mRNA levels in PBS-treated control cells (solid line—right axis). Data are presented as mean ± SEM. *p < 0.05. n = 4–6/group