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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Microglial depletion alters the brain neuroimmune response to acute binge ethanol withdrawal

Fig. 4

Acute ethanol changes BV2 microglial cytokine gene expression in vitro. Time course of cytokine expression in ethanol-treated BV2 cells: Microglia-like BV2 cells were treated with ethanol (85 mM) and the ethanol evaporated over time. Media ethanol concentrations (MECs) were measured (dashed lineleft axis) and mRNA was collected from the BV2 cells. mRNA levels of a TNFα b Ccl2 c IL-1ra, and d IL-4 in ethanol-treated cells were normalized to mRNA levels in PBS-treated control cells (solid lineright axis). Data are presented as mean ± SEM. *p < 0.05. n = 4–6/group

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