Fig. 6
From: Microglial depletion alters the brain neuroimmune response to acute binge ethanol withdrawal
Treatment with CSF1R inhibitor PLX5622 alters brain gene expression. Mice received chow containing PLX5622 for 3 weeks and mRNA was isolated from whole brain. a RT-PCR showed marked reduction in several microglial transcripts, indicating substantial microglial depletion. Other neuroimmune transcripts were also assessed. b The effects of PLX5622 on non-microglial transcripts were also assessed. Note that PLX5622 had little to no effect on neuronal, oligodendrocyte, or endothelial transcripts, but increased the astrocyte marker GFAP. Also note the marked changes in synaptic pruning genes, C3 and C1qA. Data are represented as mean ± SEM. *p < 0.05, Student’s t test, n = 6/group