Fig. 1
From: Toll-like receptor 4 in glial inflammatory responses to air pollution in vitro and in vivo
Flowchart of analysis. RNA responses of mixed glial cultures to nPM or LPS were analyzed by whole genome microarray for fold change by significance analysis of microarrays (SAM), and for shared RNA responses by weighted gene co-expression network analysis (WGCNA), given as correlation modules. Correlation modules are the clusters of correlated genes. The results from SAM and WGCNA were then analyzed for biological processes using the Gene Ontology (GO) database, and transcription factor targets (TFTs). GO output is given as nodes, which are enriched biological processes, and as networks, which are groups of connected nodes. Responding RNAs that met the criteria for fold change were analyzed for GO and TFT enrichment. For WGCNA, only modules of shared responses between nPM and LPS were examined