Fig. 1

Reduced numbers of bT_RM cells in PD-L1 KO animals. Single-cell suspensions of brain tissue obtained from MCMV-infected WT and PD-L1 KO animals were banded on a 70% percoll cushion. Brain leukocytes were labeled with Abs specific for anti-CD45-PE-Cy5, anti-KLRG1-PE-Cy7, anti-CD103-PE, and anti-CD8-BV-510 at 7, 14, and 30 dpi and analyzed using flow cytometry. CNS-derived lymphocytes were gated on CD8⁺ T cells and analyzed for KLRG1 and CD103 (a marker for tissue-resident memory T cells in the CNS). a Representative contour plots show expression profile of KLRG1 and CD103 at the indicated time points. b Pooled data present absolute number (mean ± SD) of CD103⁺ CD8⁺ T cells within the infected brains of WT and PD-L1 KO animals at indicated time points from two independent experiments using three animals per group/time points. *p < 0.01 WT versus PD-L1 KO at 14 and 30 dpi. c Immunohistochemical staining shows the distribution of tissue-resident memory cells in the hippocampus (upper panels) and brain parenchyma (lower panels) of MCMV-infected animals at 30 dpi (magnification 20×)