Fig. 4

Loss of PD-L1 or PD-1 resulted in fewer CD8⁺ T cells which co-express CD69 and CD103. Brain mononuclear cells from MCMV-infected WT, PD-L1 KO, and PD-1 KO mice were collected at 7 and 30 dpi and stained for flow cytometric analysis. CD8⁺ T cells were gated from the CD45⁺CD11b^low leukocyte population and analyzed for co-expression of CD69 and CD103. a Plots show expression of the CD69 and CD103 populations and are representative of two separate experiments using two to three animals/time point/group. b Pooled data present the percentage (mean ± SD) of co-expressed CD69 and CD103 cells among different groups of mice at the indicated time points. *p < 0.01 WT versus PD-1 or PD-L1 KO at 30 dpi **p < 0.001 WT versus PD-L1 KO for CD69⁺CD103⁺ at 30 dpi and **p < 0.001 WT versus PD-1 KO for CD69⁺CD103⁺ at 30 dpi. c Absolute numbers of CD8⁺ T cells that were CD69⁺CD103⁺ and CD69⁺CD103⁻ among infected WT, PD-L1, and PD-1 KO animals at 7 and 30 dpi are shown. **p < 0.001 WT versus PD-L1-KO and PD-1 KO for CD69⁺CD103⁺ at 30 dpi