Fig. 6

The PD-1: PD-L1 pathway promotes expression of CXCR3 on bT_RM following MCMV infection. Expression kinetics of the CXCR3 chemokine receptor on bT_RM cells. a Representative contour plots show the percentage of CXCR3 chemokine receptor expressing bT_RM cells, as assessed using flow cytometry at 7 and 30 dpi (n = 2–3 animals/group/time point from two independent experiments). b Data shown are mean ± SD percentage of CXCR3⁺CD103⁺ and CXCR3⁺CD103⁻ T cells among the WT, PD-L1 KO, and PD-1 KO animals at 7 and 30 dpi. *p < 0.01 WT versus PD-1 or PD-L1 KO at 30 dpi **p < 0.001 WT versus PD-1 KO and WT versus PD-L1 KO for CXCR3⁺CD103⁺ expression at 30 dpi. c Pooled data represent absolute numbers of CD8⁺ T cells that were CXCR3⁺CD103⁺ and CXCR3⁺CD103⁻ among infected WT, PD-L1, and PD-1 KO animals at 7 and 30 dpi. **p < 0.001 WT versus PD-L1-KO and PD-1 KO for CXCR3⁺CD103⁺ at 30 dpi