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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Activation of P2X7 receptor and NLRP3 inflammasome assembly in hippocampal glial cells mediates chronic stress-induced depressive-like behaviors

Fig. 3

CUS increases levels of ASC and activates the NLRP3 inflammasome. a, b Rats were exposed to CUS for 1, 2, and 3 weeks, respectively, and hippocampi were collected and analyzed for levels of NLRP3 and ASC using western blotting. Quantification of the changes of NLRP3 and ASC between the groups (n = 4 per group). c Co-immunoprecipitation assay was operated to determine the assembly of NLRP1/3 inflammasome in the hippocampus of rats subjected to different treatments anti-ASC antibody coupled beads or IgG control using western blotting. d, e Quantification of the protein changes of NLRP3 and cleaved caspase-3 according to the co-immunoprecipitation assay results (n = 4 per group). # p < 0.05, ### p < 0.001 compared to normal rats at the same time point

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Journal of Neuroinflammation

ISSN: 1742-2094