Experimental groups | Biochemical, histological, and functional evaluation |
---|---|
Sham operation 1.EP− + sham + siRNA-vector 2.EP− + sham + siRNA-HSP72 3.EP+ + sham + siRNA-vector 4.EP+ + sham + siRNA-HSP72 | |
1.Western blotting: HSP70 and synapsin I | |
2.Neurological motor function assay | |
3.Cerebral contusion assay | |
4.BBB permeability and brain edema assay | |
Traumatic brain injury (TBI) 1.EP− + TBI + siRNA-vector 2.EP− + TBI + siRNA-HSP72 3.EP+ + TBI + siRNA-vector 4.EP+ + TBI + siRNA-HSP72 | 5.Immunofluorescence triple staining to identify neuron apoptosis |
6.qPCR cytokines and chemokines array | |
7.NF-κB ChIP assay | |
8.IL-6 ELISA |