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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: GlyCAM1 negatively regulates monocyte entry into the optic nerve head and contributes to radiation-based protection in glaucoma

Fig. 2

Glycam1 gene and protein are up-regulated in the long term following radiation therapy. a Glycam1 gene expression following radiation therapy. Retina and choroidal tissue were collected from D2.Glycam1 −/− mice and wild-type counterparts (D2.Glycam1 +/+) at 12 months of age, and quantitative RT-PCR was performed. Untreated wild-type mice showed low (but present) levels of Glycam1 in the retina and choroidal tissue. This was significantly increased in wild-type mice that had undergone radiation therapy, especially in the choroid. As expected, Glycam1 was absent from D2.Glycam1 −/− eyes (n = 8/group). b–g GlyCAM1 protein expression following radiation therapy. Immunofluorescence staining of GlyCAM1 (red) shows no expression in non-irradiated D2 mice or D2.Glycam1 −/− eyes or irradiated D2.Glycam1 −/− eyes (b–d). However, there is robust staining of GlyCAM1 within the ONH vasculature and choroidal vasculature following radiation therapy (overview in e, choroid g, ONH vasculature h, i). There is also GlyCAM1 staining in the inner retina directly on an around cells in the ganglion cell layer (f). This expression is long-lived (tissue was sampled at 12 months). Numbers in lower corners show relative magnification. Scale bars = 50 μm (b–e), 10 μm (f–i), n = 6/group

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