Skip to main content
Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: GlyCAM1 negatively regulates monocyte entry into the optic nerve head and contributes to radiation-based protection in glaucoma

Fig. 3

GlyCAM1 in the ONH is sulfated whereas GlyCAM1 in the inner retina is un-sulfated. We tested tissue for binding of MECA-79, an antibody which is dependent on sulfation to bind to GlyCAM1. Co-localization of MECA-79 (green) and GlyCAM1 (red) determined that the GlyCAM1 present in the vasculature local to the optic nerve is sulfated (white arrows, a, b) and that the GlyCAM1 present in the inner retina and within capillaries within the optic nerve is un-sulfated (white arrows, c and inset d). Scale bars = 50 μm (a, c), 20 μm (b, d), n = 4/group

Back to article page