Fig. 2

Cellular distribution of GPR40 in the hypothalamus of mice. Tissue sections (5.0 μm) were prepared from the hypothalamic region of lean Swiss mice and were evaluated by indirect immunofluorescence staining using antibodies against GPR40 (a–c, green), NPY (a, red), POMC (b, red), and Iba1 (c, red). Nuclei were stained with DAPI (blue). In the captions, the arrows indicate cells co-expressing GPR40 and NPY (a), and GPR40 and POMC (b). Images are representative of three independent experiments