Microglia amplify inflammatory activation of astrocytes in manganese neurotoxicity

Table 1 qPCR primer sequences

Gene	Accession number	Forward primer (5′-3′)	Reverse primer (5′-3′)	Reference
β-actin	NM_00793.3	GCTCTCCTATGTTGCTCTAG	CGCTCCTTGCCAATACTC	^a
Bdnf	NM_001048139.1	TTACCTGGATGCCGCAAACAT	TGACCCACTCGCTAATACTGTC	^b
Caspase 1	NM_009807.2	ATGAATACAACCACTCGTACAC	ATCCTCCAGCAGCAACTTC	^c
Ccl2	NM_011331.2	TTAAAAACCTGGATCGGAACCAA	GCATTAGCTTCAGATTTACGGGT	^d
Ccl5	NM-013653.3	GCTGCTTTGCCTACCTCTCC	TCGAGTGACAAACACGACTGC	^d
Cd16	NM_010188.4	TTTGGACACCCAGATGTTTCAG	GTCTTCCTTGAGCACCTGGATC	^e
Cd206	NM_008625.1	TCTTTGCCTTTCCCAGTCTCC	TGACACCCAGCGGAATTTC	^e
Cd32	NM_010187.2	AATCCTGCCGTTCCTACTGATC	GTGTCACCGTGTCTTCCTTGAG	^e
Cd86	NM-019388	TTGTGTGTGTTCTGGAAACGGAG	AACTTAGAGGCTGTGTTGCTGGG	^e
Igf-1	NM_010512.4	AAAGCAGCCCGCTCTATCC	CTTCTGAGTCTTGGGCATGTCA	^b
Il-1β	NM_008361.3	GCAGCAGCACATCAACAAAG	CACGGGAAAGACACAGGTAG	^c
Il-6	NM_031168.1	GACAACTTTGGCATTGTGG	ATGCAGGGATGATGTTCTG	^b
Nos2	NM_010927.2	TCACGCTTGGG CTTGTT	CAGGTCACTTTGGTAGGATTTG	^a
Tnf	NM_013693.3	GTTGCCTGATTCTTGCTTCTG	GCCACCACTTGCTCCTAC	^c

ISSN: 1742-2094