Fig. 1

RTL342M improves clinical scores and reduces neurohistological damage in male DR*1501-Tg mice with chronic EAE. a Mean EAE daily scores of male DR*1501-Tg mice (treatment days indicated by black arrows) (left) and cumulative disease indices (right). *p ≤ 0.05, **p ≤ 0.01. Daily mean scores were analyzed by Mann-Whitney U and mean CDI by Student’s t test. b Days 20 and 63 p.i. spinal cord lumbar sections from EAE DR*1501-Tg mice were stained with luxol fast blue (LFB) and analyzed for demyelination and with toluidine blue for spinal cord damage and axon counts. Far right: CD4⁺ T cell frequencies were assessed in the ventro-lateral area of spinal cords of RTL342M- vs. vehicle-treated male mice. **p<0.01, ***p<0.001, one way ANOVA. c Correlation plots of % damage, % demyelination, and axon counts vs. CDI for male mice on day 63 post-immunization. d Mean EAE daily scores of female DR*1501-Tg mice (treatment days indicated by black arrows) (left) and cumulative disease indices (right)