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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: A role for cathepsin Z in neuroinflammation provides mechanistic support for an epigenetic risk factor in multiple sclerosis

Fig. 3

Cathepsin Z-deficiency does not affect the efficiency of MOG antigen processing or presentation in BMMØ and BMDC, or the efficiency of MOG-specific CD4+ T cell activation. a-e WT and Cat Z−/− (a, b, e) BMMØ and (c-d) BMDC were incubated with (a, c, e) MOG35–55 peptide (0, 1, 10, 25 μg/ml), or (b, d) recombinant MOG1–125 (0, 1, 10, 25 μg/ml) for 6 h before co-incubation with (a-d) MOG35–55-specific 2D2 CD4+ T cells or (e) WT or Cat Z−/− MOG35–55-specific 2D2 CD4+ T cells. Activation of WT and Cat Z−/− MOG35–55-specific 2D2 CD4+ T cells was assessed via CD4+ T cell CD69 surface expression after 16 h co-incubation with APCs (n = 3–6). Data presented as mean+/− SEM; no significant differences (unpaired Student’s t test, p > 0.05) from WT controls were observed

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