Fig. 2

Morphology, yield, and purity of primary cultured microglia obtained using mild trypsinization (E) or shaking (S). a, b Morphology of primary microglia isolated using mild trypsinization (a) or shaking (b). Scale bar = 50 μm. c, d Iba-1 immunostaining of primary microglia isolated using mild trypsinization (c) or shaking (d). Scale bar = 50 μm. e The yield of microglia cultures. n = 5. f–h Size of primary microglia isolated using mild trypsinization (f) or shaking (g). n = 5. i–n Purity of primary microglia isolated using mild trypsinization (i, l) or shaking (j, m) was determined by flow cytometry with CD11b (i–k) or CD45 (l–n) staining. n = 5. *p < 0.05