Fig. 5
From: Characteristics of primary rat microglia isolated from mixed cultures using two different methods
Morphological change (a–h) and cytokines release (i–l) of primary cultured microglia isolated using mild trypsinization (E) or shaking (S) after treatment with 20 ng/ml of IL-4, 50 ng/ml of LPS, or 20 ng/ml of IFNγ for 24 h. n = 4. Scale bar = 50 μm. m–o Reanalysis of data of cytokines release from primary cultured microglia isolated using mild trypsinization (E) or shaking (S) after treatment with 20 ng/ml of IL-4 (m), 50 ng/ml of LPS (n), or 20 ng/ml of IFNγ (o) for 24 h. Data were presented as fold changes compared with control. *p < 0.05; **p < 0.01