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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Thioredoxin-interacting protein links endoplasmic reticulum stress to inflammatory brain injury and apoptosis after subarachnoid haemorrhage

Fig. 5

GSK2656157 treatment suppressed PERK autophosphorylation and TXNIP expression; STF083010 treatment inhibited IRE1α endonuclease activity and TXNIP expression. Representative Western blot and densitometric quantification of protein band optical densities for p-PERK, p-eIF2α/eIF2α, ATF-5, ChREBP, splicing XBP1 (sXBP1) and TXNIP (a–j and Additional file 3: Figure S3 and Additional file 4: Figure S4). TXNIP expression was downregulated by GSK2656157 and STF083010 treatment. Results were analysed using the Fusion system (fx 7 Spectra, Vilber, France). Results are expressed as a percentage of the values for β-actin. */**p < 0.05 vs. sham; #/## p < 0.05 vs. SAH + DMSO. PERK protein kinase RNA-like ER kinase, IRE1α inositol-requiring enzyme-1α, DMSO dimethylsulfoxide, eIF-2α eukaryotic translation initiation factor-2α, ATF-5 activating transcription factor 5, ChREBP carbohydrate response element-binding protein, XBP1 X-box binding protein-1, TXNIP thioredoxin-interacting protein

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