Fig. 3

PLX treatment reduces expression of pro-inflammatory cytokine TNFα. RNA expression of TNFα (a) and MCP-1(b) in cerebellar samples from PLX and control SCA1 and wild-type mice with reference to control wild-type littermates and normalized to 18S rRNA. c, d Protein level of TNFα was quantified at 20 kD using Western blotting. N ≥ 3. Each dot represents one mouse, and values indicate mean ± SEM. Asterisk indicates p < 0.05 by one-way ANOVA followed by Bonferroni post hoc test, presence of n.s. indicates that p > 0.05 and that data was not significantly different and were not marked