Receptor tyrosine kinases play a significant role in human oligodendrocyte inflammation and cell death associated with the Lyme disease bacterium Borrelia burgdorferi

Table 1 Average fold change^a in chemokine/cytokine levels in the presence of specific siRNA at 48 h

Chemokine-cytokine/treatment	CCL2	CXCL8	CCL5	CXCL(1,2,3)	IL-6
Bb + 12.5 nM TLR2 siRNA	1.120^b	0.789	1.051^b	0.883	1.362
Bb + 12.5 nM TLR2 siRNA	(±0.368)	(±0.112)	(±0.176)	(±0.078)	(±0.264)
Bb + 12.5 nM TLR5 siRNA	0.769	0.685	0.489	0.810	1.164
Bb + 12.5 nM TLR5 siRNA	(±0.085)	(±0.032)	(±0.039)	(±0.024)	(±0.159)
Bb + 25 nM TLR4 siRNA	0.969	1.026	1.050	1.011	1.027^b
Bb + 25 nM TLR4 siRNA	(±0.195)	(±0.078)	(±0.060	(±0.052)	(±0.169)

^aFold change was calculated as B. burgdorferi (MOI 10:1) + control siRNA/B. burgdorferi + TLR siRNA for each experiment. Average fold-change values were calculated from the mean of three independent experiments, with standard error of the mean within parenthesis. Fold change values >1 indicate a downregulation of chemokine/cytokine levels, and values <1 indicate an increase in those levels. Values in italics or those in bold indicate statistically significant upregulation or downregulation respectively, in a majority (>67% or 2/3) of experiments
^bInconclusive results

ISSN: 1742-2094