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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: MicroRNA-101a regulates microglial morphology and inflammation

Fig. 4

The expression level of miR-101a in vitro and in vivo. a Mixed glial cells were divided into CD11b+ cells or CD11b− cells using CD11b-conjugated magnetic beads. The relative expression levels of miR-101a were analyzed by quantitative PCR and were compared with peritoneal macrophage (PM) and bone marrow-derived macrophages (BMDM). b CNS cells from C57BL/6J mice were divided into CD11b+ cells or CD11b− cells with the same method. c The effects of lipopolysaccharide and poly I:C on the expression of miR-101a in MG6 immortalized microglial cells. d miR-101a expression in E18, postnatal d8, and 6 weeks brains. The expression levels of miR-101a were analyzed by quantitative PCR (n = 4) and were shown as relative expression to sno202. Data are presented as means ± S.D. *P < 0.05, **P < 0.01 by Student’s t test or ANOVA followed by Tukey’s post hoc test

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