Fig. 3

Genetic deletion of PDE3A and inflammation-induced CMH development: Significant increase in grossly visible surface CMH in the LPS-treated WT and LPS-treated PDE3A KO mice compared with their respective saline controls (a). Significant increase in H&E-positive parenchymal CMH number (b), size (c), and area (d) in the LPS-treated WT and LPS-treated PDE3A KO mice compared with their respective saline controls. Representative images of H&E-positive CMH in saline- and LPS-treated WT and PDE3A^−/− KO mice (e). Data are presented as mean ± SEM. Statistical test: one-way ANOVA with Bonferroni’s post-test or Kruskal-Wallis test with Dunn’s post-test for parenchymal CMH and one-sample t test with a hypothesized mean = 0 for surface CMH. *p < 0.05, **p < 0.01, ***p < 0.001