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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Thrombin-induced, TNFR-dependent miR-181c downregulation promotes MLL1 and NF-κB target gene expression in human microglia

Fig. 1

Thrombin downregulates miR-181c in human microglia. a Heatmap of the miRNA array identifying differential miRNA candidates. miRNAs exhibiting significantly altered expression between thrombin-treated and untreated control conditions were identified with red shading representing downregulation and green shading representing upregulation. Experiments performed in triplicate. b miRNAs exhibiting ≥1.5 × downregulation in thrombin-treated microglia were selected for qRT-PCR validation. For each miRNA candidate, qRT-PCR measurements were performed to obtain a mean CT value for each sample. CT values of the different samples were compared using the 2−ΔΔCT method with GAPDH expression levels used as an internal reference. All results presented as means ± SEMs from three independent experiments. *p < 0.05, downregulation versus control

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