Fig. 2

Thrombin-driven, PAR4-mediated TNF-α expression downregulates miR-181c and upregulates MLL1. a qRT-PCR comparing relative expression levels of TNF-α, miR-181c, and MLL1 at 1, 3, 6, and 12 h after 24-h thrombin treatment. Control indicates untreated control microglia. b qRT-PCR comparing relative expression levels of TNF-α, miR-181c, and MLL1 at 3 h following treatment with thrombin, PAR4AP, or PAR1AP. *p < 0.05 versus control, †p < 0.05 versus thrombin, ‡p < 0.05 versus PAR4AP. c, d qRT-PCR comparing relative expression levels of c miR-181c and d MLL1 at 3 h following treatment with thrombin or PAR4AP after blocking TNF-α or silencing TNFR1/2. *p < 0.05 versus wild-type microglia, †p < 0.05 versus anti-TNF-α microglia. All results presented as means ± SEMs from three independent experiments. *p < 0.05 versus control