Table 2 Oligonucleotide primer sets used for RT-qPCR
From: Acute neuroinflammation induces AIS structural plasticity in a NOX2-dependent manner
Gene | Accession no. | Forward primer | Reverse primer |
|---|---|---|---|
Tnf-α | NM_013693.1 | 5′-GCCCACGTCGTAGCAAACCACC-3′ | 5′-CCCATCGGCTGGCACCACTA-3′ |
COX-2 (Ptgs2) | NM_009367.1 | 5′-TTGCTGGCCGGGTTGCTGG-3′ | 5′-CAGGGAGAAGCGTTTGCGGT-3′ |
NOX2 (Cybb) | NM_023965.1 | 5′-GGGAACTGGGCTGTGAATGA-3′ | 5′-CAGTGCTGACCCAAGGAGTT-3′ |
Mrc1 | NM_008625.2 | 5′-GGCTGATTACGAGCAGTGGA-3′ | 5′-CATCACTCCAGGTGAACCCC-3′ |
Fizz-1 (Retnlb) | NM_020509.3 | 5′-CAGCTGATGGTCCCAGTGAAT-3′ | 5′-AGTGGAGGGATAGTTAGCTGG-3′ |
TGF-β | NM_009367.2 | 5′-CTCCCCTCCGAAAATGCCA-3′ | 5′-GTTTTGCAAGCGGAAGACCC-3′ |
Cyclophilin A | NM_008907.1 | 5′-CTAGAGGGCATGGATGTGGT-3′ | 5′-TGACATCCTTCAGTGGCTTG-3′ |
PGK1 | NM_008828.3 | 5′-ATGCAAAGACTGGCCAAGCTA C-3′ | 5′-AGCCACAGCCTCAGCATATTTC-3′ |