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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: B cells do not have a major pathophysiologic role in acute ischemic stroke in mice

Fig. 2

Lack of B cells does not impact stroke outcome in a transient middle cerebral artery occlusion (tMCAO) model. a Representative 2,3,5-triphenyltetrazolium chloride stains of three corresponding brain sections and infarct volumes of control (WT) and B cell-deficient (JHD −/−) mice at days 1 (left) and 3 (right) after 60-min tMCAO (n = 9–11/group). Infarct volumes are similar between the two groups for both time points (unpaired, two-tailed Student’s t test). b Left, representative immunocytologic staining of CD11b+ and Ly6B+ cells within the ischemic brains of WT and JHD −/− mice. b Right, quantification revealed comparable numbers of CD11b+ monocytes and Ly6B+ neutrophils in the ipsilesional hemispheres of WT and JHD −/− mice at days 1 (n = 6/group) and 3 (n = 4–5/group) after tMCAO (unpaired, two-tailed Student’s t test). c Left, representative brain sections from a WT and a JHD −/− mouse stained for the neuronal marker NeuN and subjected to TUNEL assay to visualize apoptosis. Quantification of dead neurons per optical field in basal ganglial as well as cortical regions at days 1 (n = 6/group) and 3 (n = 5/group) was comparable between WT and JHD −/− mice (unpaired, two-tailed Student’s t test). c Right, representative immunocytologic staining of glial fibrillary acidic protein (GFAP) immunoreactivity in the penumbra (black lines) of the ischemic cortex of a WT and a JHD −/− mouse at day 3 after tMCAO (top). Representative anti-GFAP Western blot analysis (cc cortex contralesional, ci cortex ipsilesional, bgc basal ganglia contralesional, bgi basal ganglia ipsilesional) and densitometric quantification of ipsilesional GFAP protein expression in the basal ganglial as well as cortical regions at day 3 (n = 5/group) after tMCAO (bottom) was comparable between WT and JHD −/− mice (unpaired, two-tailed Student’s t test). d Relative gene expression of tumor necrosis factor α (TNFα), interleukin (IL)-1β, and IL-10 in the ischemic basal ganglia and cortex are similar at day 1 after tMCAO of WT and JHD −/− mice (n = 5–6/group) when normalized to sham operation (unpaired, two-tailed Student’s t test)

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