Fig. 3

Prestroke B cell adoptive transfer (AT) into Rag1 ^−/− mice does not worsen stroke outcome in a transient middle cerebral artery occlusion (tMCAO) model. Left, representative 2,3,5-­triphenyltetrazolium chloride stains of three corresponding brain sections of a C57BL/6 mouse (WT), a Rag1 ^−/− mouse without adoptive cell transfer (Rag1 ^-/-) and with adoptively transferred B cells (Rag1 ^−/−> AT B cells) or T cells (Rag1 ^−/− AT B cells) on day 1 after 60-min tMCAO. Right, infarct volumes are reduced in Rag1 ^−/− mice (n = 10) compared with those in WT mice (n = 6). Only AT of T cells (n = 8), but not B cells (n = 9), increased infarct volumes (one-way analysis of variance with post hoc Bonferroni adjustment for P values). ***P < 0.001